[Download] "Media Composition Influences Growth, Enzyme Activity and Virulence of the Entomopathogen Metarhizium Anisopliae (Hypocreales: Clavicipitaceae) (Report)" by Pakistan Journal of Zoology * eBook PDF Kindle ePub Free
eBook details
- Title: Media Composition Influences Growth, Enzyme Activity and Virulence of the Entomopathogen Metarhizium Anisopliae (Hypocreales: Clavicipitaceae) (Report)
- Author : Pakistan Journal of Zoology
- Release Date : January 31, 2010
- Genre: Life Sciences,Books,Science & Nature,
- Pages : * pages
- Size : 214 KB
Description
Byline: Jianhui Wu, Shaukat Ali, Zhen Huang, Shunxiang Ren and Shujia Cai - Email:renxncn@yahoo.com.cn, - alientomologist@gmail.com. Abstract.- Three isolates of Metarhizium anisopliae were cultured on six media having different compositions on the basis of chitin, carbon and nitrogen contents. The effect of nutrition on growth and virulence was studied by measuring colony growth, spore yield, germination rate, spore bound Pr1, lipase activity and virulence of inoculum produced by different media against 2nd instar larvae of diamondback moth (Plutella xylostella). Chitin peptone nutrition media produced the highest colony growth but maximum conidial yield was observed for medium having high C/N ratio. Highest lipase activity (3.63 umol/ml/min) was observed for spores produced by High C/N medium for isolates M408 while highest Pr1 activity (2.1umol/ml/min) was observed on CPN medium for M460. Conidia from medium C/N ratio proved to be the most virulent for all the three isolates with Median survival time (ST50) values of 1.45, 1.62 and 1.72 days for M408, M440 and M460, respectively.